S-nitrosylation of thaumatin-like protein in Ziziphus jujuba and its roles in chilling stress and pathogen stress responses

Yizhou Gao; Ting Guo; Lingling Liu; Li Li; Zhengbao Wu; Yunhong Jiang; Zhaojun Ban; Zhen Yang

doi:10.1016/j.postharvbio.2025.113782

S-nitrosylation of thaumatin-like protein in Ziziphus jujuba and its roles in chilling stress and pathogen stress responses

Yizhou Gao, Ting Guo, Lingling Liu, Li Li, Zhengbao Wu, Yunhong Jiang, Zhaojun Ban^*, Zhen Yang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Nitric oxide is a key regulator in alleviating chilling injury in postharvest fruits through S-nitrosylation of functional proteins. This study identified ZjTLP, a thaumatin-like protein in jujube, as a critical target of S-nitrosylation at cysteine residues Cys31, Cys195, and Cys212. Through bioinformatics and molecular simulations, we revealed structural stabilization and increased solvent accessibility of ZjTLP protein under S-nitrosylation. Functional assays demonstrated ZjTLP's β-1,3-glucanase activity and its extracellular secretion properties. ZjTLP gene expression was modulated by NO treatment, low-temperature stress, and fungal infection, highlighting its potential role in cold stress mitigation and pathogen defense.

Original language	English
Article number	113782
Number of pages	13
Journal	Postharvest Biology and Technology
Volume	230
Early online date	16 Jul 2025
DOIs	https://doi.org/10.1016/j.postharvbio.2025.113782
Publication status	Published - 1 Dec 2025

Keywords

Chilling injury
Jujube
Nitric oxide
S-nitrosylation
Thaumatin-like protein

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10.1016/j.postharvbio.2025.113782

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title = "S-nitrosylation of thaumatin-like protein in Ziziphus jujuba and its roles in chilling stress and pathogen stress responses",

abstract = "Nitric oxide is a key regulator in alleviating chilling injury in postharvest fruits through S-nitrosylation of functional proteins. This study identified ZjTLP, a thaumatin-like protein in jujube, as a critical target of S-nitrosylation at cysteine residues Cys31, Cys195, and Cys212. Through bioinformatics and molecular simulations, we revealed structural stabilization and increased solvent accessibility of ZjTLP protein under S-nitrosylation. Functional assays demonstrated ZjTLP's β-1,3-glucanase activity and its extracellular secretion properties. ZjTLP gene expression was modulated by NO treatment, low-temperature stress, and fungal infection, highlighting its potential role in cold stress mitigation and pathogen defense.",

keywords = "Chilling injury, Jujube, Nitric oxide, S-nitrosylation, Thaumatin-like protein",

author = "Yizhou Gao and Ting Guo and Lingling Liu and Li Li and Zhengbao Wu and Yunhong Jiang and Zhaojun Ban and Zhen Yang",

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doi = "10.1016/j.postharvbio.2025.113782",

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TY - JOUR

T1 - S-nitrosylation of thaumatin-like protein in Ziziphus jujuba and its roles in chilling stress and pathogen stress responses

AU - Gao, Yizhou

AU - Guo, Ting

AU - Liu, Lingling

AU - Li, Li

AU - Wu, Zhengbao

AU - Jiang, Yunhong

AU - Ban, Zhaojun

AU - Yang, Zhen

PY - 2025/12/1

Y1 - 2025/12/1

N2 - Nitric oxide is a key regulator in alleviating chilling injury in postharvest fruits through S-nitrosylation of functional proteins. This study identified ZjTLP, a thaumatin-like protein in jujube, as a critical target of S-nitrosylation at cysteine residues Cys31, Cys195, and Cys212. Through bioinformatics and molecular simulations, we revealed structural stabilization and increased solvent accessibility of ZjTLP protein under S-nitrosylation. Functional assays demonstrated ZjTLP's β-1,3-glucanase activity and its extracellular secretion properties. ZjTLP gene expression was modulated by NO treatment, low-temperature stress, and fungal infection, highlighting its potential role in cold stress mitigation and pathogen defense.

AB - Nitric oxide is a key regulator in alleviating chilling injury in postharvest fruits through S-nitrosylation of functional proteins. This study identified ZjTLP, a thaumatin-like protein in jujube, as a critical target of S-nitrosylation at cysteine residues Cys31, Cys195, and Cys212. Through bioinformatics and molecular simulations, we revealed structural stabilization and increased solvent accessibility of ZjTLP protein under S-nitrosylation. Functional assays demonstrated ZjTLP's β-1,3-glucanase activity and its extracellular secretion properties. ZjTLP gene expression was modulated by NO treatment, low-temperature stress, and fungal infection, highlighting its potential role in cold stress mitigation and pathogen defense.

KW - Chilling injury

KW - Jujube

KW - Nitric oxide

KW - S-nitrosylation

KW - Thaumatin-like protein

UR - https://www.scopus.com/pages/publications/105010617491

U2 - 10.1016/j.postharvbio.2025.113782

DO - 10.1016/j.postharvbio.2025.113782

M3 - Article

AN - SCOPUS:105010617491

SN - 0925-5214

VL - 230

JO - Postharvest Biology and Technology

JF - Postharvest Biology and Technology

M1 - 113782

ER -

S-nitrosylation of thaumatin-like protein in Ziziphus jujuba and its roles in chilling stress and pathogen stress responses

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Keywords

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