Sarcopenic muscle extracellular matrix dictates muscle adaptations in a 3D-engineered muscle model of COPD

Efpraxia Kritikaki*, Ioannis Vogiatzis, Gerasimos Terzis, Davina C M Simoes

*Corresponding author for this work

Research output: Contribution to journalMeeting Abstractpeer-review


Introduction: We investigated whether exercie-induced extracellular matrix (ECM) protein expression from sarcopenic COPD can replicate muscle wasting and loss of muscle strength in a 3D-engineered muscle.

Methods: Exercise-induced changes in ECM composition in sarcopenic COPD muscle (FFMI; 15.6 Kg.cm2, n=10) and healthy people (FFMI; 19.4 Kg.cm2, n=18) were replicated in a xeno-free human 3D-engineered muscle tissue organoid. Human myoblasts (Lonza) embedded in specific ECM composition from COPD sarcopenic and healthy people were used to construct a 3D-muscle model. Muscle fibre width, and protein expression was analysed using immune fluorescence (IF) images. Gene expression was quantified using RT-PCR.

Results: Compared to healthy, treatment with increasing amount of ECM proteins from sarcopenic COPD induced muscle fibre wasting and failure in myoblast cell attachment and differentiation (Figure 1). Sarcopenic COPD compared to healthy ECM composition produced myofibers with shorter length and smaller width evidenced by IF analyses of anti-sarcomeric α-actinin and anti-desmin staining. Myogenic and growth factors were also affected by ECM compositionn from sarcopenic COPD.

Conclusion: Compared to healthy, muscle from sarcopenic COPD presents particular ECM composition that does not favour exercise-induced muscle growth.
Original languageEnglish
Article numberPA1800
Number of pages1
JournalEuropean Respiratory Journal
Issue numbersuppl 67
Publication statusPublished - 9 Sept 2023
EventERS International Congress 2023 - Milan, Italy
Duration: 9 Sept 202313 Sept 2023

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