Segmentation of retinal ganglion cells from fluorescent microscopy imaging

Silvia Baglietto, Ibolya E. Kepiro, Gerrit Hilgen, Evelyne Sernagor, Vittorio Murino, Diego Sona

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

The visual information processing starts in the retina. The working mechanisms of its complex stratified circuits, in which ganglion cells play a central role, is still largely unknown. Understanding the visual coding is a challenging and active research area also requiring automated analysis of retinal images. It demands appropriate algorithms and methods for studying a network population of strictly entangled cells. Within this framework, we propose a combined technique for segmenting retinal ganglion cell (RGC) bodies, the output elements of the retina. The method incorporates a blob enhancement filtering in order to select the specific cell shapes, an active contour process for precise border segmentation and a watershed transform step which separates single cell contours in possible grouped segmentations. The proposed approach has been validated on fluorescent microscopy images of mouse retinas with promising results.

Original languageEnglish
Title of host publicationBIOIMAGING 2017 - 4th International Conference on Bioimaging, Proceedings
Subtitle of host publicationPart of 10th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2017
EditorsAna Fred, Margarida Silveira, Hugo Gamboa, Mario Vaz
PublisherScitepress
Pages17-23
Number of pages7
ISBN (Electronic)9789897582158
DOIs
Publication statusPublished - 2017
Externally publishedYes
Event4th International Conference on Bioimaging, BIOIMAGING 2017 - Part of 10th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2017 - Porto, Portugal
Duration: 21 Feb 201723 Feb 2017

Publication series

NameBIOIMAGING 2017 - 4th International Conference on Bioimaging, Proceedings; Part of 10th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2017
Volume2017-January

Conference

Conference4th International Conference on Bioimaging, BIOIMAGING 2017 - Part of 10th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2017
CountryPortugal
CityPorto
Period21/02/1723/02/17

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