Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase

David Forrest, Katherine James, Yulia Yuzenkova, Nikolay Zenkin

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11 Citations (Scopus)
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Abstract

Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a B400 kDa five-subunit catalytic core. Here we characterize a hypothetical B100 kDa single-chain protein, YonO, encoded by the SPb prophage of Bacillus subtilis. YonO shares very distant homology with msRNAPs, but no homology with single-subunit polymerases. We show that despite homology to only a few amino acids of msRNAP, and the absence of most of the
conserved domains, YonO is a highly processive DNA-dependent RNA polymerase. We demonstrate that YonO is a bona fide RNAP of the SPb bacteriophage that specifically transcribes its late genes, and thus represents a novel type of bacteriophage RNAPs. YonO and related proteins present in various bacteria and bacteriophages have diverged from msRNAPs before the Last Universal Common Ancestor, and, thus, may resemble the single-subunit ancestor of all msRNAPs.
Original languageEnglish
Article number15774
Number of pages8
JournalNature Communications
Volume8
Issue number1
DOIs
Publication statusPublished - 6 Jun 2017
Externally publishedYes

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