Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function

Nicholas Kwiatkowski, Nannette Jelluma, Panagis Filippakopoulos, Meera Soundararajan, Michael Manak, Mijung Kwon, Hwan Geun Choi, Taebo Sim, Quinn Deveraux, Sabine Rottmann, David Pellman, Jagesh Shah, Geert Kops, Stefan Knapp, Nathanael Gray

Research output: Contribution to journalArticlepeer-review

187 Citations (Scopus)


Mps1, a dual-specificity kinase, is required for the proper functioning of the spindle assembly checkpoint and for the maintenance of chromosomal stability. As Mps1 function has been implicated in numerous phases of the cell cycle, the development of a potent, selective small-molecule inhibitor of Mps1 should facilitate dissection of Mps1-related biology. We describe the cellular effects and Mps1 cocrystal structures of new, selective small-molecule inhibitors of Mps1. Consistent with RNAi studies, chemical inhibition of Mps1 leads to defects in Mad1 and Mad2 establishment at unattached kinetochores, decreased Aurora B kinase activity, premature mitotic exit and gross aneuploidy, without any evidence of centrosome duplication defects. However, in U2OS cells having extra centrosomes (an abnormality found in some cancers), Mps1 inhibition increases the frequency of multipolar mitoses. Lastly, Mps1 inhibitor treatment resulted in a decrease in cancer cell viability.
Original languageEnglish
Pages (from-to)359-368
JournalNature Chemical Biology
Issue number5
Publication statusPublished - 2010


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