Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1

C. Martinez-Fleites; Nicola L. Smith; Johan Turkenburg; Gary Black; Edward Taylor

doi:10.1107/S1744309109032813

Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1

C. Martinez-Fleites, Nicola L. Smith, Johan Turkenburg, Gary Black, Edward Taylor

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively. The truncated forms crystallized in a hexagonal space group, forming a trimer around the threefold crystallographic axis. The arrangement of the fold is very similar to that observed in the structure of the related hyaluronate lyase HylP1. The structural elements putatively involved in substrate recognition are found to be conserved in both the HylP2 and HylP3 fragments.

Original language	English
Pages (from-to)	963-966
Journal	Acta Crystallographica Section F:Structural Biology Communications
Volume	65
Issue number	10
DOIs	https://doi.org/10.1107/S1744309109032813
Publication status	Published - 2009

Keywords

carbohydrates
lyases
streptococci
bacteriophages.

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10.1107/S1744309109032813

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title = "Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1",

abstract = "The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 {\AA} resolution, respectively. The truncated forms crystallized in a hexagonal space group, forming a trimer around the threefold crystallographic axis. The arrangement of the fold is very similar to that observed in the structure of the related hyaluronate lyase HylP1. The structural elements putatively involved in substrate recognition are found to be conserved in both the HylP2 and HylP3 fragments.",

keywords = "carbohydrates, lyases, streptococci, bacteriophages.",

author = "C. Martinez-Fleites and Smith, \{Nicola L.\} and Johan Turkenburg and Gary Black and Edward Taylor",

year = "2009",

doi = "10.1107/S1744309109032813",

language = "English",

volume = "65",

pages = "963--966",

journal = "Acta Crystallographica Section F:Structural Biology Communications",

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T1 - Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1

AU - Martinez-Fleites, C.

AU - Smith, Nicola L.

AU - Turkenburg, Johan

AU - Black, Gary

AU - Taylor, Edward

PY - 2009

Y1 - 2009

N2 - The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively. The truncated forms crystallized in a hexagonal space group, forming a trimer around the threefold crystallographic axis. The arrangement of the fold is very similar to that observed in the structure of the related hyaluronate lyase HylP1. The structural elements putatively involved in substrate recognition are found to be conserved in both the HylP2 and HylP3 fragments.

AB - The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 Å resolution, respectively. The truncated forms crystallized in a hexagonal space group, forming a trimer around the threefold crystallographic axis. The arrangement of the fold is very similar to that observed in the structure of the related hyaluronate lyase HylP1. The structural elements putatively involved in substrate recognition are found to be conserved in both the HylP2 and HylP3 fragments.

KW - carbohydrates

KW - lyases

KW - streptococci

KW - bacteriophages.

UR - https://www.scopus.com/pages/publications/70350318920

U2 - 10.1107/S1744309109032813

DO - 10.1107/S1744309109032813

M3 - Article

SN - 1744-3091

VL - 65

SP - 963

EP - 966

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

IS - 10

ER -

Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1

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