Substrate Concentration and Plasmid Transfer Frequency between Bacteria in a Model Rhizosphere

David Pearce, Michael Bazin, James Lynch

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The influence of substrate concentration on plasmid transfer frequency in the rhizosphere was investigated using a physical model employing a hollow fiber membrane instead of a real root. The absolute number of transconjugants produced increased with increasing substrate (glucose) concentration, but the plasmid transfer frequency decreased exponentially with increasing substrate concentration from 4.4 × 10-3 at 90 μg ml-1 glucose to 1.35 × 10-5 at 3600 μg ml-1 glucose. These results were found to be heavily dependant on donor to recipient ratio and distribution of strains, but independent of initial donor and recipient inoculum density and regime. These observations also show that plasmid transfer frequency is reduced at high substrate concentrations, which suggests that plasmid transfer is either stimulated when growth limiting nutrient is scarce or inhibited when it is abundant.
Original languageEnglish
Pages (from-to)57-63
JournalMicrobial Ecology
Volume40
Issue number1
DOIs
Publication statusPublished - Jul 2000

Fingerprint Dive into the research topics of 'Substrate Concentration and Plasmid Transfer Frequency between Bacteria in a Model Rhizosphere'. Together they form a unique fingerprint.

Cite this