TY - JOUR
T1 - Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology
AU - Anderson, Rosaleen
AU - Groundwater, Paul
AU - Huang, Yongxue
AU - James, Arthur
AU - Orenga, Sylvain
AU - Rigby, Annette
AU - Roger-Dalbert, Céline
AU - Perry, John
PY - 2008
Y1 - 2008
N2 - The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.
AB - The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.
KW - microorganism detection
KW - microorganism differentiation
KW - clinical bacteriology
KW - diagnostic reagent
KW - chromogenic peptidase substrate
KW - 9-(4′-Aminophenyl)-10-methylacridinium salt
KW - alanine aminopeptidase
KW - β-Alanine aminopeptidase
KW - cystic fibrosis
U2 - 10.1016/j.bmcl.2007.11.018
DO - 10.1016/j.bmcl.2007.11.018
M3 - Article
SN - 0960-894X
SN - 1464-3405
VL - 18
SP - 832
EP - 835
JO - Bioorganic & Medicinal Chemistry Letters
JF - Bioorganic & Medicinal Chemistry Letters
IS - 2
ER -
