Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology

Rosaleen Anderson; Paul Groundwater; Yongxue Huang; Arthur James; Sylvain Orenga; Annette Rigby; Céline Roger-Dalbert; John Perry

doi:10.1016/j.bmcl.2007.11.018

Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology

Rosaleen Anderson, Paul Groundwater, Yongxue Huang, Arthur James, Sylvain Orenga, Annette Rigby, Céline Roger-Dalbert, John Perry

Northumbria University

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.

Original language	English
Pages (from-to)	832-835
Journal	Bioorganic & Medicinal Chemistry Letters
Volume	18
Issue number	2
DOIs	https://doi.org/10.1016/j.bmcl.2007.11.018
Publication status	Published - 2008

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

microorganism detection
microorganism differentiation
clinical bacteriology
diagnostic reagent
chromogenic peptidase substrate
9-(4′-Aminophenyl)-10-methylacridinium salt
alanine aminopeptidase
β-Alanine aminopeptidase
cystic fibrosis

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10.1016/j.bmcl.2007.11.018

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Anderson, R., Groundwater, P., Huang, Y., James, A., Orenga, S., Rigby, A., Roger-Dalbert, C., & Perry, J. (2008). Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology. Bioorganic & Medicinal Chemistry Letters, 18(2), 832-835. https://doi.org/10.1016/j.bmcl.2007.11.018

@article{3578ba8392254b62a0e296110f884978,

title = "Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology",

abstract = "The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.",

keywords = "microorganism detection, microorganism differentiation, clinical bacteriology, diagnostic reagent, chromogenic peptidase substrate, 9-(4′-Aminophenyl)-10-methylacridinium salt, alanine aminopeptidase, β-Alanine aminopeptidase, cystic fibrosis",

author = "Rosaleen Anderson and Paul Groundwater and Yongxue Huang and Arthur James and Sylvain Orenga and Annette Rigby and C{\'e}line Roger-Dalbert and John Perry",

year = "2008",

doi = "10.1016/j.bmcl.2007.11.018",

language = "English",

volume = "18",

pages = "832--835",

journal = "Bioorganic \& Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier",

number = "2",

}

Anderson, R, Groundwater, P, Huang, Y, James, A, Orenga, S, Rigby, A, Roger-Dalbert, C & Perry, J 2008, 'Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology', Bioorganic & Medicinal Chemistry Letters, vol. 18, no. 2, pp. 832-835. https://doi.org/10.1016/j.bmcl.2007.11.018

Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology. / Anderson, Rosaleen; Groundwater, Paul; Huang, Yongxue et al.
In: Bioorganic & Medicinal Chemistry Letters, Vol. 18, No. 2, 2008, p. 832-835.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology

AU - Anderson, Rosaleen

AU - Groundwater, Paul

AU - Huang, Yongxue

AU - James, Arthur

AU - Orenga, Sylvain

AU - Rigby, Annette

AU - Roger-Dalbert, Céline

AU - Perry, John

PY - 2008

Y1 - 2008

N2 - The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.

AB - The synthesis and initial evaluation of novel chromogenic substrates with potential in the detection and differentiation of cultured bacterial colonies are described. The substrates were readily hydrolysed by specific aminopeptidase activity to release the chromogen, 9-(4′-aminophenyl)-10-methylacridinium salt, which provided a clear visual indication of the presence of the corresponding bacteria.

KW - microorganism detection

KW - microorganism differentiation

KW - clinical bacteriology

KW - diagnostic reagent

KW - chromogenic peptidase substrate

KW - 9-(4′-Aminophenyl)-10-methylacridinium salt

KW - alanine aminopeptidase

KW - β-Alanine aminopeptidase

KW - cystic fibrosis

UR - https://www.scopus.com/pages/publications/38349070369

U2 - 10.1016/j.bmcl.2007.11.018

DO - 10.1016/j.bmcl.2007.11.018

M3 - Article

SN - 0960-894X

SN - 1464-3405

VL - 18

SP - 832

EP - 835

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

IS - 2

ER -

Synthesis and evaluation of novel chromogenic peptidase substrates based on 9-(4′-aminophenyl)-10-methylacridinium salts as diagnostic tools in clinical bacteriology

Abstract

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Keywords

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