Synthesis and Initial Evaluation of a Novel Fluorophore for Selective FMDV 3C Protease Detection

Samerah Malik, Alex Sinclair, Ali Ryan, Adam Le Gresley

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Abstract

The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.
Original languageEnglish
Article number3599
JournalMolecules
Volume25
Issue number16
DOIs
Publication statusPublished - 7 Aug 2020

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