Temperature acclimation of marine crabs: Changes in plasma membrane fluidity and lipid composition

Mirela Cuculescu; David Hyde; Ken Bowler

doi:10.1016/0306-4565(94)00058-Q

Temperature acclimation of marine crabs: Changes in plasma membrane fluidity and lipid composition

Mirela Cuculescu^*, David Hyde, Ken Bowler

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

1. 1. Crabs were acclimated for 3 weeks to cold and warm temperatures. Plasma membranes prepared from leg muscle were analysed for fluidity and lipid composition. 2. 2. Plasma membranes from cold-acclimated crabs were more fluid and had lower cholesterol to phospholipid ratios than those from warm-acclimated crabs. The ratio saturated/unsaturated fatty acids was little altered by temperature acclimation. 3. 3. Plasma membranes from the stenothermal species (Cancer pagurus) were slightly more fluid (at DPH probe level) and had lower cholesterol/phospholipid ratios than those from the eurythermal species (Carcinus maenas). 4. 4. Plasma membranes from crabs acclimated in spring had more saturated and less polyunsaturated fatty acids than those from crabs acclimated in autumn.

Original language	English
Pages (from-to)	207-222
Number of pages	16
Journal	Journal of Thermal Biology
Volume	20
Issue number	1-2
DOIs	https://doi.org/10.1016/0306-4565(94)00058-Q
Publication status	Published - 1995
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

acclimation
cholesterol
crabs
eurythermal
fatty acids
fluidity
phospholipid
plasma membrane
seasonal variation
stenothermal
Temperature

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10.1016/0306-4565(94)00058-Q

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title = "Temperature acclimation of marine crabs: Changes in plasma membrane fluidity and lipid composition",

abstract = "1. 1. Crabs were acclimated for 3 weeks to cold and warm temperatures. Plasma membranes prepared from leg muscle were analysed for fluidity and lipid composition. 2. 2. Plasma membranes from cold-acclimated crabs were more fluid and had lower cholesterol to phospholipid ratios than those from warm-acclimated crabs. The ratio saturated/unsaturated fatty acids was little altered by temperature acclimation. 3. 3. Plasma membranes from the stenothermal species (Cancer pagurus) were slightly more fluid (at DPH probe level) and had lower cholesterol/phospholipid ratios than those from the eurythermal species (Carcinus maenas). 4. 4. Plasma membranes from crabs acclimated in spring had more saturated and less polyunsaturated fatty acids than those from crabs acclimated in autumn.",

keywords = "acclimation, cholesterol, crabs, eurythermal, fatty acids, fluidity, phospholipid, plasma membrane, seasonal variation, stenothermal, Temperature",

author = "Mirela Cuculescu and David Hyde and Ken Bowler",

note = "Funding Information: Acknowledgements-The work presented in this paper was funded by a grant from the Leverhulme Trust. Many thanks to Dr Andrew R. Cossins for the use of the equipment for the fluorescence polarization measurements and helpful advice. We thank Tim Pearson for help with crab maintenance. We also thank Mr Frank Allen (fisherman, Hartlepool) for supplying crabs and Mr Tony Fergurson (Steetley, Hartlepool) for supplying filtered sea water.",

year = "1995",

doi = "10.1016/0306-4565(94)00058-Q",

language = "English",

volume = "20",

pages = "207--222",

journal = "Journal of Thermal Biology",

issn = "0306-4565",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Temperature acclimation of marine crabs

T2 - Changes in plasma membrane fluidity and lipid composition

AU - Cuculescu, Mirela

AU - Hyde, David

AU - Bowler, Ken

N1 - Funding Information: Acknowledgements-The work presented in this paper was funded by a grant from the Leverhulme Trust. Many thanks to Dr Andrew R. Cossins for the use of the equipment for the fluorescence polarization measurements and helpful advice. We thank Tim Pearson for help with crab maintenance. We also thank Mr Frank Allen (fisherman, Hartlepool) for supplying crabs and Mr Tony Fergurson (Steetley, Hartlepool) for supplying filtered sea water.

PY - 1995

Y1 - 1995

N2 - 1. 1. Crabs were acclimated for 3 weeks to cold and warm temperatures. Plasma membranes prepared from leg muscle were analysed for fluidity and lipid composition. 2. 2. Plasma membranes from cold-acclimated crabs were more fluid and had lower cholesterol to phospholipid ratios than those from warm-acclimated crabs. The ratio saturated/unsaturated fatty acids was little altered by temperature acclimation. 3. 3. Plasma membranes from the stenothermal species (Cancer pagurus) were slightly more fluid (at DPH probe level) and had lower cholesterol/phospholipid ratios than those from the eurythermal species (Carcinus maenas). 4. 4. Plasma membranes from crabs acclimated in spring had more saturated and less polyunsaturated fatty acids than those from crabs acclimated in autumn.

AB - 1. 1. Crabs were acclimated for 3 weeks to cold and warm temperatures. Plasma membranes prepared from leg muscle were analysed for fluidity and lipid composition. 2. 2. Plasma membranes from cold-acclimated crabs were more fluid and had lower cholesterol to phospholipid ratios than those from warm-acclimated crabs. The ratio saturated/unsaturated fatty acids was little altered by temperature acclimation. 3. 3. Plasma membranes from the stenothermal species (Cancer pagurus) were slightly more fluid (at DPH probe level) and had lower cholesterol/phospholipid ratios than those from the eurythermal species (Carcinus maenas). 4. 4. Plasma membranes from crabs acclimated in spring had more saturated and less polyunsaturated fatty acids than those from crabs acclimated in autumn.

KW - acclimation

KW - cholesterol

KW - crabs

KW - eurythermal

KW - fatty acids

KW - fluidity

KW - phospholipid

KW - plasma membrane

KW - seasonal variation

KW - stenothermal

KW - Temperature

UR - https://www.scopus.com/pages/publications/0000061347

U2 - 10.1016/0306-4565(94)00058-Q

DO - 10.1016/0306-4565(94)00058-Q

M3 - Article

AN - SCOPUS:0000061347

SN - 0306-4565

VL - 20

SP - 207

EP - 222

JO - Journal of Thermal Biology

JF - Journal of Thermal Biology

IS - 1-2

ER -

Temperature acclimation of marine crabs: Changes in plasma membrane fluidity and lipid composition

Abstract

UN SDGs

Keywords

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