Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer

Ricardo D C Ribeiro, Deepali Pal, David Jamieson, Kenneth S Rankin, Matthew Benning, Kenneth W Dalgarno, Ana M Ferreira

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)
5 Downloads (Pure)

Abstract

Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(l-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblasts. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1-2 h, with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell coating in enabling reliable bioprinting is also presented.

Original languageEnglish
Pages (from-to)12967-12974
Number of pages8
JournalACS applied materials & interfaces
Volume9
Issue number15
Early online date21 Mar 2017
DOIs
Publication statusPublished - 19 Apr 2017

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