TY - JOUR
T1 - Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer
AU - Da Conceicao Ribeiro, Ricardo
AU - Pal, Deepali
AU - Jamieson, David
AU - Rankin, Kenneth S.
AU - Benning, Matthew
AU - Dalgarno, Kenneth W.
AU - Ferreira, Ana Marina
N1 - Funding information: The work reported in this article was partially funded by the Arthritis Research UK Tissue Engineering Centre (19429) and the EPSRC Centre for Innovative Manufacture in Medical Devices (MeDe Innovation; EP/K029592). R.D.C.R. acknowledges support from Newcastle University for his Ph.D. studentship. D.P. was supported by a JGW Patterson Foundation Fellowship and an NC3Rs Training Fellowship. The authors also thank T. Booth for the confocal microscopy image acquisition at the Bioimaging Unit (Newcastle University, U.K.), K. White for help in interpretation of the TEM images at the Electron Microscopy Research Services (Newcastle University, U.K.), and G. Cuthbert for karyotype analyses at the Institute of Genetic Medicine (Newcastle University, U.K.).
PY - 2017/4/19
Y1 - 2017/4/19
N2 - Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(l-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblasts. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1-2 h, with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell coating in enabling reliable bioprinting is also presented.
AB - Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(l-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblasts. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1-2 h, with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell coating in enabling reliable bioprinting is also presented.
KW - Capsules
KW - Cations
KW - Cell Line
KW - Polylysine
KW - Polymers/chemistry
UR - https://www.scopus.com/pages/publications/85018516049
U2 - 10.1021/acsami.6b16434
DO - 10.1021/acsami.6b16434
M3 - Article
C2 - 28323412
SN - 1944-8244
VL - 9
SP - 12967
EP - 12974
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
IS - 15
ER -
