The adhesion G-protein-coupled receptor mayo/CG11318 controls midgut development in Drosophila

Fernando Vieira Contreras; Genevieve M. Auger; Lena Müller; Vincent Richter; Wolf Huetteroth; Florian Seufert; Peter W. Hildebrand; Nicole Scholz; Andreas S. Thum; Dmitrij Ljaschenko; Beatriz Blanco-Redondo; Tobias Langenhan

doi:10.1016/j.celrep.2023.113640

The adhesion G-protein-coupled receptor mayo/CG11318 controls midgut development in Drosophila

Fernando Vieira Contreras, Genevieve M. Auger, Lena Müller, Vincent Richter, Wolf Huetteroth, Florian Seufert, Peter W. Hildebrand, Nicole Scholz, Andreas S. Thum, Dmitrij Ljaschenko, Beatriz Blanco-Redondo^*, Tobias Langenhan^*

^*Corresponding author for this work

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Abstract

Adhesion G-protein-coupled receptors (aGPCRs) form a large family of cell surface molecules with versatile tasks in organ development. Many aGPCRs still await their functional and pharmacological deorphanization. Here, we characterized the orphan aGPCR CG11318/mayo of Drosophila melanogaster and found it expressed in specific regions of the gastrointestinal canal and anal plates, epithelial specializations that control ion homeostasis. Genetic removal of mayo results in tachycardia, which is caused by hyperkalemia of the larval hemolymph. The hyperkalemic effect can be mimicked by a raise in ambient potassium concentration, while normal potassium levels in mayo^KO mutants can be restored by pharmacological inhibition of potassium channels. Intriguingly, hyperkalemia and tachycardia are caused non-cell autonomously through mayo-dependent control of enterocyte proliferation in the larval midgut, which is the primary function of this aGPCR. These findings characterize the ancestral aGPCR Mayo as a homeostatic regulator of gut development.

Original language	English
Article number	113640
Number of pages	20
Journal	Cell Reports
Volume	43
Issue number	1
Early online date	4 Jan 2024
DOIs	https://doi.org/10.1016/j.celrep.2023.113640
Publication status	Published - 23 Jan 2024
Externally published	Yes

Keywords

adhesion GPCR
cardiac physiology
CP: Developmental biology
Drosophila melanogaster
GAIN domain
gut
potassium homeostasis

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10.1016/j.celrep.2023.113640Licence: CC BY-NC-ND

1-s2.0-S2211124723016510-mainFinal published version, 4.45 MBLicence: CC BY-NC-ND

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Vieira Contreras, F., Auger, G. M., Müller, L., Richter, V., Huetteroth, W., Seufert, F., Hildebrand, P. W., Scholz, N., Thum, A. S., Ljaschenko, D., Blanco-Redondo, B., & Langenhan, T. (2024). The adhesion G-protein-coupled receptor mayo/CG11318 controls midgut development in Drosophila. Cell Reports, 43(1), Article 113640. https://doi.org/10.1016/j.celrep.2023.113640

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AU - Seufert, Florian

AU - Hildebrand, Peter W.

AU - Scholz, Nicole

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AU - Blanco-Redondo, Beatriz

AU - Langenhan, Tobias

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The adhesion G-protein-coupled receptor mayo/CG11318 controls midgut development in Drosophila

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