The core phageome and its interrelationship with preterm human milk lipids

Wen C. Yew; Gregory R. Young; Andrew Nelson; William Cheung; Christopher J. Stewart; Simon H. Bridge; Claire Granger; Janet E. Berrington; Nicholas D. Embleton; Darren L. Smith

doi:10.1016/j.celrep.2023.113373

The core phageome and its interrelationship with preterm human milk lipids

Wen C. Yew, Gregory R. Young, Andrew Nelson, William Cheung, Christopher J. Stewart, Simon H. Bridge, Claire Granger, Janet E. Berrington, Nicholas D. Embleton, Darren L. Smith^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

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Abstract

Phages and lipids in human milk (HM) may benefit preterm infant health by preventing gastrointestinal pathobiont overgrowth and microbiome modulation. Lipid association may promote vertical transmission of phages to the infant. Despite this, interrelationships between lipids and phages are poorly characterized in preterm HM. Shotgun metagenomics and untargeted lipidomics of phage and lipid profiles from 99 preterm HM samples reveals that phages are abundant and prevalent from the first week and throughout the first 100 days of lactation. Phage-host richness of preterm HM increases longitudinally. Core phage communities characterized by Staphylococcus- and Propionibacterium-infecting phages are significantly correlated with long-chain fatty acid abundances over lactational age. We report here a phage-lipid interaction in preterm HM, highlighting the potential importance of phage carriage in preterm HM. These results reveal possible strategies for phage carriage in HM and their importance in early-life microbiota development.

Original language	English
Article number	113373
Pages (from-to)	1-16
Number of pages	1
Journal	Cell Reports
Volume	42
Issue number	11
Early online date	16 Nov 2023
DOIs	https://doi.org/10.1016/j.celrep.2023.113373
Publication status	Published - 28 Nov 2023

Keywords

preterm infants
microbiome
human milk
bacteriophages
lipids
breastfeeding
neonatal nutrition
lipidomics
virome

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title = "The core phageome and its interrelationship with preterm human milk lipids",

abstract = "Phages and lipids in human milk (HM) may benefit preterm infant health by preventing gastrointestinal pathobiont overgrowth and microbiome modulation. Lipid association may promote vertical transmission of phages to the infant. Despite this, interrelationships between lipids and phages are poorly characterized in preterm HM. Shotgun metagenomics and untargeted lipidomics of phage and lipid profiles from 99 preterm HM samples reveals that phages are abundant and prevalent from the first week and throughout the first 100 days of lactation. Phage-host richness of preterm HM increases longitudinally. Core phage communities characterized by Staphylococcus- and Propionibacterium-infecting phages are significantly correlated with long-chain fatty acid abundances over lactational age. We report here a phage-lipid interaction in preterm HM, highlighting the potential importance of phage carriage in preterm HM. These results reveal possible strategies for phage carriage in HM and their importance in early-life microbiota development.",

keywords = "preterm infants, microbiome, human milk, bacteriophages, lipids, breastfeeding, neonatal nutrition, lipidomics, virome",

author = "Yew, \{Wen C.\} and Young, \{Gregory R.\} and Andrew Nelson and William Cheung and Stewart, \{Christopher J.\} and Bridge, \{Simon H.\} and Claire Granger and Berrington, \{Janet E.\} and Embleton, \{Nicholas D.\} and Smith, \{Darren L.\}",

note = "Funding information: This project is funded by Action Medical Research (AMR) for children (grant reference no. GN2730).",

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doi = "10.1016/j.celrep.2023.113373",

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T1 - The core phageome and its interrelationship with preterm human milk lipids

AU - Yew, Wen C.

AU - Young, Gregory R.

AU - Nelson, Andrew

AU - Cheung, William

AU - Stewart, Christopher J.

AU - Bridge, Simon H.

AU - Granger, Claire

AU - Berrington, Janet E.

AU - Embleton, Nicholas D.

AU - Smith, Darren L.

N1 - Funding information: This project is funded by Action Medical Research (AMR) for children (grant reference no. GN2730).

PY - 2023/11/28

Y1 - 2023/11/28

N2 - Phages and lipids in human milk (HM) may benefit preterm infant health by preventing gastrointestinal pathobiont overgrowth and microbiome modulation. Lipid association may promote vertical transmission of phages to the infant. Despite this, interrelationships between lipids and phages are poorly characterized in preterm HM. Shotgun metagenomics and untargeted lipidomics of phage and lipid profiles from 99 preterm HM samples reveals that phages are abundant and prevalent from the first week and throughout the first 100 days of lactation. Phage-host richness of preterm HM increases longitudinally. Core phage communities characterized by Staphylococcus- and Propionibacterium-infecting phages are significantly correlated with long-chain fatty acid abundances over lactational age. We report here a phage-lipid interaction in preterm HM, highlighting the potential importance of phage carriage in preterm HM. These results reveal possible strategies for phage carriage in HM and their importance in early-life microbiota development.

AB - Phages and lipids in human milk (HM) may benefit preterm infant health by preventing gastrointestinal pathobiont overgrowth and microbiome modulation. Lipid association may promote vertical transmission of phages to the infant. Despite this, interrelationships between lipids and phages are poorly characterized in preterm HM. Shotgun metagenomics and untargeted lipidomics of phage and lipid profiles from 99 preterm HM samples reveals that phages are abundant and prevalent from the first week and throughout the first 100 days of lactation. Phage-host richness of preterm HM increases longitudinally. Core phage communities characterized by Staphylococcus- and Propionibacterium-infecting phages are significantly correlated with long-chain fatty acid abundances over lactational age. We report here a phage-lipid interaction in preterm HM, highlighting the potential importance of phage carriage in preterm HM. These results reveal possible strategies for phage carriage in HM and their importance in early-life microbiota development.

KW - preterm infants

KW - microbiome

KW - human milk

KW - bacteriophages

KW - lipids

KW - breastfeeding

KW - neonatal nutrition

KW - lipidomics

KW - virome

UR - https://www.scopus.com/pages/publications/85176414078

U2 - 10.1016/j.celrep.2023.113373

DO - 10.1016/j.celrep.2023.113373

M3 - Article

SN - 2211-1247

VL - 42

SP - 1

EP - 16

JO - Cell Reports

JF - Cell Reports

IS - 11

M1 - 113373

ER -

The core phageome and its interrelationship with preterm human milk lipids

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