The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Silvia A. Synowsky, Sally L. Shirran, Fiona G. M. Cooke, Antony N. Antoniou, Catherine H. Botting, Simon J. Powis

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Extracellular vesicles (EVs) are released by most cell types and have been associated with multiple immunomodulatory functions. MHC class I molecules have crucial roles in antigen presentation and in eliciting immune responses and are known to be incorporated into EVs. However, the MHC class I immunopeptidome of EVs has not been established. Here, using a small-scale immunoisolation of the antigen serotypes HLA-A*02:01 and HLA-B*27:05 expressed on the Epstein-Barr virus-transformed B cell line Jesthom and MS of the eluted peptides from both cells and EVs, we identified 516 peptides that bind either HLA-A*02:01 or HLA-B*27:05. Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not significantly differ between the peptide pools isolated from cells or EVs, indicating that during EV biogenesis, no obvious editing of the MHC class I immunopeptidome occurs. These results, for the first time, establish EVs as a source of MHC class I peptides that can be used for the study of the immunopeptidome and in the discovery of potential neoantigens for immunotherapies.
Original languageEnglish
Pages (from-to)17084-17092
JournalJournal of Biological Chemistry
Issue number41
Early online date31 Aug 2017
Publication statusPublished - 13 Oct 2017


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