The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

Nong Gao; Malcolm von Schantz; Russell G. Foster; Jim Hardie

doi:10.1016/S0022-1910(99)00082-7

The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

Nong Gao, Malcolm von Schantz, Russell G. Foster, Jim Hardie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.

Original language	English
Pages (from-to)	1011-1019
Number of pages	9
Journal	Journal of Insect Physiology
Volume	45
Issue number	11
Early online date	10 Aug 1999
DOIs	https://doi.org/10.1016/S0022-1910(99)00082-7
Publication status	Published - 1 Nov 1999
Externally published	Yes

Access to Document

10.1016/S0022-1910(99)00082-7

Cite this

@article{62092b48dffe43bbb2e8e92f795cc3d1,

title = "The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae",

abstract = "In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.",

keywords = "Aphid, Arrestin, Brain photoreceptors, Extraretinal photoreceptors, Immunocytochemistry, Light microscopy, Megoura viciae, Opsin",

author = "Nong Gao and {von Schantz}, Malcolm and Foster, {Russell G.} and Jim Hardie",

note = "Funding information: This study was supported by the Biotechnology and Biological Sciences Research Council, UK. The authors thank Drs J. Bentrop, W. J. DeGrip, R. Paulsen, P. R{\"o}hlich, J. Saari and S. Subramaniam for the generous gift of antibodies.",

year = "1999",

month = nov,

day = "1",

doi = "10.1016/S0022-1910(99)00082-7",

language = "English",

volume = "45",

pages = "1011--1019",

journal = "Journal of Insect Physiology",

issn = "0022-1910",

publisher = "Elsevier",

number = "11",

}

TY - JOUR

T1 - The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

AU - Gao, Nong

AU - von Schantz, Malcolm

AU - Foster, Russell G.

AU - Hardie, Jim

N1 - Funding information: This study was supported by the Biotechnology and Biological Sciences Research Council, UK. The authors thank Drs J. Bentrop, W. J. DeGrip, R. Paulsen, P. Röhlich, J. Saari and S. Subramaniam for the generous gift of antibodies.

PY - 1999/11/1

Y1 - 1999/11/1

N2 - In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.

AB - In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.

KW - Aphid

KW - Arrestin

KW - Brain photoreceptors

KW - Extraretinal photoreceptors

KW - Immunocytochemistry

KW - Light microscopy

KW - Megoura viciae

KW - Opsin

UR - http://www.scopus.com/inward/record.url?scp=0032789935&partnerID=8YFLogxK

U2 - 10.1016/S0022-1910(99)00082-7

DO - 10.1016/S0022-1910(99)00082-7

M3 - Article

AN - SCOPUS:0032789935

VL - 45

SP - 1011

EP - 1019

JO - Journal of Insect Physiology

JF - Journal of Insect Physiology

SN - 0022-1910

IS - 11

ER -

The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

Abstract

Access to Document

Other files and links

Fingerprint

Cite this