The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

Nong Gao; Malcolm von Schantz; Russell G. Foster; Jim Hardie

doi:10.1016/S0022-1910(99)00082-7

The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

Nong Gao, Malcolm von Schantz, Russell G. Foster, Jim Hardie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.

Original language	English
Pages (from-to)	1011-1019
Number of pages	9
Journal	Journal of Insect Physiology
Volume	45
Issue number	11
Early online date	10 Aug 1999
DOIs	https://doi.org/10.1016/S0022-1910(99)00082-7
Publication status	Published - 1 Nov 1999
Externally published	Yes

Keywords

Aphid
Arrestin
Brain photoreceptors
Extraretinal photoreceptors
Immunocytochemistry
Light microscopy
Megoura viciae
Opsin

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10.1016/S0022-1910(99)00082-7

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title = "The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae",

abstract = "In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.",

keywords = "Aphid, Arrestin, Brain photoreceptors, Extraretinal photoreceptors, Immunocytochemistry, Light microscopy, Megoura viciae, Opsin",

author = "Nong Gao and {von Schantz}, Malcolm and Foster, {Russell G.} and Jim Hardie",

note = "Funding information: This study was supported by the Biotechnology and Biological Sciences Research Council, UK. The authors thank Drs J. Bentrop, W. J. DeGrip, R. Paulsen, P. R{\"o}hlich, J. Saari and S. Subramaniam for the generous gift of antibodies.",

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N1 - Funding information: This study was supported by the Biotechnology and Biological Sciences Research Council, UK. The authors thank Drs J. Bentrop, W. J. DeGrip, R. Paulsen, P. Röhlich, J. Saari and S. Subramaniam for the generous gift of antibodies.

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AB - In an attempt to identify the brain photoreceptors that mediate the photoperiodic response of the vetch aphid, Megoura viciae, we utilised immunocytochemical techniques and employed 20 antibodies directed against invertebrate and vertebrate opsins and phototransduction proteins. A sub-set of these antibodies (to Drosophila rhodopsin 1: RH1-1; vertebrate cone opsins: COS-1; CERN-874; CERN-933; vertebrate rod opsin: CERN-901; vertebrate arrestin: AB-Arr; vertebrate transducin+arrestin+rhodopsin kinase+cGMP phosphodiesterase: CERN-911; and vertebrate cellular retinoid binding protein: CRALBP) consistently labelled an anterior ventral neuropile region of the protocerebrum. These anatomical findings, coupled with previous localised illumination and micro-lesion studies, provide strong evidence that this region of the aphid brain houses the photoperiodic photoreceptors. The present study also confirms that the medial (Group I) neurosecretory cells are not the photoperiodic photoreceptors.

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The putative brain photoperiodic photoreceptors in the vetch aphid, Megoura viciae

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Keywords

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