TY - JOUR
T1 - Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri
AU - Yi, Yue
AU - Dolfing, Jan
AU - Jin, Ge
AU - Fang, XiaoYu
AU - Han, WenHao
AU - Liu, LaiYan
AU - Tang, YueQin
AU - Cheng, Lei
N1 - Funding information: This study was supported by National Natural Science Foundation of China (no. 92051108), Agricultural Science and Technology Innovation Project of the Chinese Academy of Agriculture Science (no. CAAS-ASTIP-2016-BIOMA), the Central Public-interest Scientific Institution Basal Research Fund (Y2021PT02, Y2021XK06, 2013ZL001), Hainan Science and Technology Program (ZDYF2021XDNY300).
PY - 2023/4/1
Y1 - 2023/4/1
N2 - Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H /CO , and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH tolerances of M. barkeri fed with acetate, H /CO , and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion. [Abstract copyright: Copyright © 2023 Elsevier Ltd. All rights reserved.]
AB - Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H /CO , and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH tolerances of M. barkeri fed with acetate, H /CO , and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion. [Abstract copyright: Copyright © 2023 Elsevier Ltd. All rights reserved.]
KW - Ammonia tolerance
KW - Thermodynamic restriction
KW - Methanosarcina barkeri
KW - Gibbs free energy
UR - http://www.scopus.com/inward/record.url?scp=85148630974&partnerID=8YFLogxK
U2 - 10.1016/j.watres.2023.119664
DO - 10.1016/j.watres.2023.119664
M3 - Article
C2 - 36775717
SN - 0043-1354
VL - 232
SP - 1
EP - 7
JO - Water Research
JF - Water Research
M1 - 119664
ER -