Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri

Yue Yi; Jan Dolfing; Ge Jin; XiaoYu Fang; WenHao Han; LaiYan Liu; YueQin Tang; Lei Cheng

doi:10.1016/j.watres.2023.119664

Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri

Yue Yi, Jan Dolfing, Ge Jin, XiaoYu Fang, WenHao Han, LaiYan Liu, YueQin Tang^*, Lei Cheng^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H /CO , and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH tolerances of M. barkeri fed with acetate, H /CO , and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion. [Abstract copyright: Copyright © 2023 Elsevier Ltd. All rights reserved.]

Original language	English
Article number	119664
Pages (from-to)	1-7
Number of pages	7
Journal	Water Research
Volume	232
Early online date	24 Jan 2023
DOIs	https://doi.org/10.1016/j.watres.2023.119664
Publication status	Published - 1 Apr 2023

Keywords

Ammonia tolerance
Thermodynamic restriction
Methanosarcina barkeri
Gibbs free energy

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10.1016/j.watres.2023.119664

NU upload 2023 WR Ammonia accepted copy_Accepted author manuscript, 1.5 MBLicence: CC BY-NC-ND

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title = "Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri",

abstract = "Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H /CO , and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH tolerances of M. barkeri fed with acetate, H /CO , and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion. [Abstract copyright: Copyright {\textcopyright} 2023 Elsevier Ltd. All rights reserved.]",

keywords = "Ammonia tolerance, Thermodynamic restriction, Methanosarcina barkeri, Gibbs free energy",

author = "Yue Yi and Jan Dolfing and Ge Jin and XiaoYu Fang and WenHao Han and LaiYan Liu and YueQin Tang and Lei Cheng",

note = "Funding information: This study was supported by National Natural Science Foundation of China (no. 92051108), Agricultural Science and Technology Innovation Project of the Chinese Academy of Agriculture Science (no. CAAS-ASTIP-2016-BIOMA), the Central Public-interest Scientific Institution Basal Research Fund (Y2021PT02, Y2021XK06, 2013ZL001), Hainan Science and Technology Program (ZDYF2021XDNY300).",

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doi = "10.1016/j.watres.2023.119664",

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TY - JOUR

T1 - Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri

AU - Yi, Yue

AU - Dolfing, Jan

AU - Jin, Ge

AU - Fang, XiaoYu

AU - Han, WenHao

AU - Liu, LaiYan

AU - Tang, YueQin

AU - Cheng, Lei

N1 - Funding information: This study was supported by National Natural Science Foundation of China (no. 92051108), Agricultural Science and Technology Innovation Project of the Chinese Academy of Agriculture Science (no. CAAS-ASTIP-2016-BIOMA), the Central Public-interest Scientific Institution Basal Research Fund (Y2021PT02, Y2021XK06, 2013ZL001), Hainan Science and Technology Program (ZDYF2021XDNY300).

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H /CO , and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH tolerances of M. barkeri fed with acetate, H /CO , and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion. [Abstract copyright: Copyright © 2023 Elsevier Ltd. All rights reserved.]

AB - Ammonia is a ubiquitous potential inhibitor of anaerobic digestion processes, mainly exhibiting inhibition towards methanogenic activity. However, knowledge as to how ammonia affects the methanogens is still limited. In this study, we cultured a multitrophic methanogen, Methanosarcina barkeri DSM 800, with acetate, H /CO , and methanol to evaluate the influence of ammonia on different methanogenic pathways. Aceticlastic methanogenesis was more sensitive to increased ammonia concentrations than hydrogenotrophic and methylotrophic methanogenesis. Theoretical maximum NH tolerances of M. barkeri fed with acetate, H /CO , and methanol were calculated to be 39.1 ± 9.0, 104.3 ± 7.4, and 85.7 ± 1.0 mg/L, respectively. The order of the ΔG range of M. barkeri under three methanogenic pathways reflected the order of ammonia tolerance of M. barkeri. Our results provide insights into the role of the thermodynamic potential of methanogenesis on the tolerance of ammonia stress; and shed light on the mechanism of ammonia inhibition on anaerobic digestion. [Abstract copyright: Copyright © 2023 Elsevier Ltd. All rights reserved.]

KW - Ammonia tolerance

KW - Thermodynamic restriction

KW - Methanosarcina barkeri

KW - Gibbs free energy

UR - https://www.scopus.com/pages/publications/85148630974

U2 - 10.1016/j.watres.2023.119664

DO - 10.1016/j.watres.2023.119664

M3 - Article

C2 - 36775717

SN - 0043-1354

VL - 232

SP - 1

EP - 7

JO - Water Research

JF - Water Research

M1 - 119664

ER -

Thermodynamic restrictions determine ammonia tolerance of methanogenic pathways in Methanosarcina barkeri

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