TY - JOUR
T1 - Thermodynamically favorable reactions shape the archaeal community affecting bacterial community assembly in oil reservoirs
AU - Zhao, Jie-Yu
AU - Hu, Bing
AU - Dolfing, Jan
AU - Li, Yan
AU - Tang, Yue-Qin
AU - Jiang, Yiming
AU - Chi, Chang-Qiao
AU - Xing, Jianmin
AU - Nie, Yong
AU - Wu, Xiao-Lei
N1 - Funding information: The study was supported by the National Key R&D Program of China (2018YFA0902100 and 2018YFA0902103 to XLW), the National Natural Science Foundation of China (31770120 to XLW), and Open Funding Project of the State Key Laboratory of Biochemical Engineering (No. 2020KF-05).
PY - 2021/8/10
Y1 - 2021/8/10
N2 - Microbial community assembly mechanisms are pivotal for understanding the ecological functions of microorganisms in biogeochemical cycling in Earth's ecosystems, yet rarely investigated in the context of deep terrestrial ecology. Here, the microbial communities in the production waters collected from water injection wells and oil production wells across eight oil reservoirs throughout northern China were determined and analyzed by proportional distribution analysis and null model analysis. A ‘core’ microbiota consisting of three bacterial genera, including Arcobacter, Pseudomonas and Acinetobacter, and eight archaeal genera, including Archaeoglobus, Methanobacterium, Methanothermobacter, unclassified Methanobacteriaceae, Methanomethylovorans, Methanoculleus, Methanosaeta and Methanolinea, was found to be present in all production water samples. Canonical correlation analysis reflected that the core archaea were significantly influenced by temperature and reservoir depth, while the core bacteria were affected by the combined impact of the core archaea and environmental factors. Thermodynamic calculations indicate that bioenergetic constraints are the driving force that governs the enrichment of two core archaeal guilds, aceticlastic methanogens versus hydrogenotrophic methanogens, in low- and high-temperature oil reservoirs, respectively. Collectively, our study indicates that microbial community structures in wells of oil reservoirs are structured by the thermodynamic window of opportunity, through which the core archaeal communities are accommodated directly followed by the deterministic recruiting of core bacterial genera, and then the stochastic selection of some other microbial members from local environments. Our study enhances the understanding of the microbial assembly mechanism in deep terrestrial habitats. Meanwhile, our findings will support the development of functional microbiota used for bioremediation and bioaugmentation in microbial enhanced oil recovery.
AB - Microbial community assembly mechanisms are pivotal for understanding the ecological functions of microorganisms in biogeochemical cycling in Earth's ecosystems, yet rarely investigated in the context of deep terrestrial ecology. Here, the microbial communities in the production waters collected from water injection wells and oil production wells across eight oil reservoirs throughout northern China were determined and analyzed by proportional distribution analysis and null model analysis. A ‘core’ microbiota consisting of three bacterial genera, including Arcobacter, Pseudomonas and Acinetobacter, and eight archaeal genera, including Archaeoglobus, Methanobacterium, Methanothermobacter, unclassified Methanobacteriaceae, Methanomethylovorans, Methanoculleus, Methanosaeta and Methanolinea, was found to be present in all production water samples. Canonical correlation analysis reflected that the core archaea were significantly influenced by temperature and reservoir depth, while the core bacteria were affected by the combined impact of the core archaea and environmental factors. Thermodynamic calculations indicate that bioenergetic constraints are the driving force that governs the enrichment of two core archaeal guilds, aceticlastic methanogens versus hydrogenotrophic methanogens, in low- and high-temperature oil reservoirs, respectively. Collectively, our study indicates that microbial community structures in wells of oil reservoirs are structured by the thermodynamic window of opportunity, through which the core archaeal communities are accommodated directly followed by the deterministic recruiting of core bacterial genera, and then the stochastic selection of some other microbial members from local environments. Our study enhances the understanding of the microbial assembly mechanism in deep terrestrial habitats. Meanwhile, our findings will support the development of functional microbiota used for bioremediation and bioaugmentation in microbial enhanced oil recovery.
KW - Core archaea
KW - Microbial community assembly
KW - Microbiome
KW - Oil reservoirs
KW - Thermodynamic constraint
UR - http://www.scopus.com/inward/record.url?scp=85103378347&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2021.146506
DO - 10.1016/j.scitotenv.2021.146506
M3 - Article
SN - 0048-9697
VL - 781
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 146506
ER -