Tracking Circulating HLA-Specific IgG-Producing Memory B Cells with the B-Cell ImmunoSpot Assay

Delphine Kervella, Sebastiaan Heidt, Robert Fairchild, Stephen Todryk*, Oriol Bestard

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Donor-specific antibodies (DSA) against human leukocyte antigen (HLA) molecules are a major risk factor for rejection of transplanted organs (in antibody-mediated rejection [ABMR]), particularly in patients who have prior sensitization or receive insufficient immunosuppression through minimization or noncompliance. These DSA are measured routinely in the serum of patients prior to transplantation mainly using bead-based technologies or cell-based assays. However, the absence of detectable serum DSA does not always reflect the absence of sensitization or histologically defined ABMR, and so it has been proposed that the detection and measurement of memory B cells capable of secreting antibodies against donor HLA antigens could be carried out using B-cell ImmunoSpot, to better inform the degree of immune sensitization of transplant patients prior to as well as after transplantation. Such an assay is described here.

Original languageEnglish
Title of host publicationHandbook of ELISPOT
Subtitle of host publicationMethods and Protocols
EditorsAlexander E. Kalyuzhny
Place of PublicationNew York
Number of pages9
ISBN (Electronic)9781071636909
ISBN (Print)9781071636893, 9781071636923
Publication statusPublished - 19 Mar 2024

Publication series

NameMethods in Molecular Biology (MIMB)
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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