Ultrahigh-sensitivity label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection

Ling Chen, Yuankui Leng, Bin Liu, Juan Liu, Sheng-Peng Wan, Tao Wu, Jinhui Yuan, Liyang Shao, Guoqiang Gu, Yong Qing Fu, Hengyi Xu, Yonghua Xiong, Xing-Dao He, Qiang Wu

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

An ultra-high sensitivity label-free optical fiber biosensor for inactivated Staphylococcus aureus (S. aureus) detection is proposed and investigated in this study, with additional advantages of robust and stability compared to traditional tapered fiber structure. The proposed fiber biosensor is based on a tapered singlemode- no core-singlemode fiber coupler (SNSFC) structure, where the no core fiber was tapered to small diameter (taper-waist diameter of about 10 µm) and functionalized with the pig immunoglobulin G (IgG) antibody for detection of S. aureus. The measured maximum wavelength shift of the sensor for an S. aureus concentration of 7 × 101 CFU/ml (colony forming unit per milliliter) is 2.04 nm, which is equivalent to a limit of detection (LOD) of 3.1 CFU/ml (a highest LOD reported so far for optical fiber biosensors), considering the maximum wavelength variation of the sensor in phosphate buffered saline (PBS) is ±0.03 nm over 40 minutes, where 3 times of maximum wavelength variation (3 × 0.03 = 0.09 nm) is defined as measurement limit. The response time of the developed fiber sensor is less than 30 minutes. The ultra-sensitive biosensor has potential to be widely applied to various areas such as disease, medical diagnostics and food safety inspection.
Original languageEnglish
Article number128283
Number of pages7
JournalSensors and Actuators B: Chemical
Volume320
Early online date22 May 2020
DOIs
Publication statusPublished - 1 Oct 2020

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