Ultrahigh-sensitivity label-free single mode-tapered multimode-single mode fiber U-shaped biosensor for Staphylococcus aureus detection

Shi Qiu, Yuankui Leng, Jinhui Yuan*, Zhichao Zhang, Xian Zhou, Bin Liu*, Chao Mei, Binbin Yan, Kuiru Wang, Xinzhu Sang, Keping Long, Chongxiu Yu, Xingdao He, Gerald Farrell, Qiang Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The rapid detection of Staphylococcus aureus (S. aureus), particularly in the case of very low levels of S. aureus, is very important in food safety and medical diagnostics. In this paper, a fast (<20 mins), label-free biosensor using a U-shaped single mode- tapered multimode- single mode (STMS) fiber structure is presented. The average wavelength shift of the sensor (modified with the IgG from porcine serum with a concentration 200 μg/mL) is as high as 1.552 nm when it is immersed into an S. aureus sample with a concentration of 4 cells/mL. By further diluting the S. aureus to 0.4 cells/mL for a sample volume of 1.5 mL, it is found that a dip wavelength shift of 0.348 nm is observed on occasion which confirms that the biosensor can detect a single colony (cell) of S. aureus. Results for the detection of S. aureus in milk and lettuce samples are also presented and are compared with the wavelength shift results in phosphate buffered saline (PBS), and it is found that the variation in the average wavelength shift is − 21% for S. aureus concentrations from 4 to 4 × 103 cells/mL.
Original languageEnglish
Article number132927
Number of pages8
JournalSensors and Actuators B: Chemical
Volume375
Early online date1 Nov 2022
DOIs
Publication statusE-pub ahead of print - 1 Nov 2022

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