Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

Sana Rauf; Hafiz Imran Ahmad Qazi; Jingting Luo; Chen Fu; Ran Tao; Sajid Rauf; Lei Yang; Honglang Li; Yongqing Fu

doi:10.3390/chemosensors9110311

Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

Sana Rauf, Hafiz Imran Ahmad Qazi, Jingting Luo^*, Chen Fu, Ran Tao, Sajid Rauf, Lei Yang, Honglang Li, Yongqing Fu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

19 Downloads (Pure)

Abstract

We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.

Original language	English
Article number	311
Pages (from-to)	1-13
Number of pages	13
Journal	Chemosensors
Volume	9
Issue number	11
DOIs	https://doi.org/10.3390/chemosensors9110311
Publication status	Published - 4 Nov 2021

Keywords

leaky surface acoustic wave (LSAW)
immunosensor
AFP
MoS2@Cu2O-Au
gold staining
Leaky surface acoustic wave (LSAW)
Immunosensor
MoS @Cu O-Au
Gold staining

Access to Document

10.3390/chemosensors9110311Licence: CC BY

chemosensors-09-00311Final published version, 2.62 MBLicence: CC BY

Cite this

@article{1223502197c6471e987ca225a7a9efc4,

title = "Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids",

abstract = "We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.",

keywords = "leaky surface acoustic wave (LSAW), immunosensor, AFP, MoS2@Cu2O-Au, gold staining, Leaky surface acoustic wave (LSAW), Immunosensor, MoS @Cu O-Au, Gold staining",

author = "Sana Rauf and Qazi, \{Hafiz Imran Ahmad\} and Jingting Luo and Chen Fu and Ran Tao and Sajid Rauf and Lei Yang and Honglang Li and Yongqing Fu",

note = "Funding information: This work was financially supported by the Key Research and Development Program of Guangdong Province (Grant No.: 2020B0101040002), Special Projects in Key Fields of Colleges in Guangdong Province (2020ZDZX2097), Research Project in Fundamental and Application Fields of Guangdong Province (2020A1515110561), Guangdong Basic and Applied Basic Research Foundation (2019A1515111199), and Shenzhen Science \& Technology Project (Grant No. JCYJ20180507182106754, JCYJ20180507182439574, RCBS20200714114918249, GJHZ20200073109583010), the Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), International Exchange Grant (IEC/NSFC/201078) through Royal Society and the National Natural Science Foundation of China, and EPSRC NetworkPlus in Digitalised Surface.",

year = "2021",

month = nov,

day = "4",

doi = "10.3390/chemosensors9110311",

language = "English",

volume = "9",

pages = "1--13",

journal = "Chemosensors",

issn = "2227-9040",

publisher = "MDPI AG",

number = "11",

}

TY - JOUR

T1 - Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

AU - Rauf, Sana

AU - Qazi, Hafiz Imran Ahmad

AU - Luo, Jingting

AU - Fu, Chen

AU - Tao, Ran

AU - Rauf, Sajid

AU - Yang, Lei

AU - Li, Honglang

AU - Fu, Yongqing

N1 - Funding information: This work was financially supported by the Key Research and Development Program of Guangdong Province (Grant No.: 2020B0101040002), Special Projects in Key Fields of Colleges in Guangdong Province (2020ZDZX2097), Research Project in Fundamental and Application Fields of Guangdong Province (2020A1515110561), Guangdong Basic and Applied Basic Research Foundation (2019A1515111199), and Shenzhen Science & Technology Project (Grant No. JCYJ20180507182106754, JCYJ20180507182439574, RCBS20200714114918249, GJHZ20200073109583010), the Engineering and Physical Sciences Research Council of UK (EPSRC EP/P018998/1), International Exchange Grant (IEC/NSFC/201078) through Royal Society and the National Natural Science Foundation of China, and EPSRC NetworkPlus in Digitalised Surface.

PY - 2021/11/4

Y1 - 2021/11/4

N2 - We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.

AB - We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.

KW - leaky surface acoustic wave (LSAW)

KW - immunosensor

KW - AFP

KW - MoS2@Cu2O-Au

KW - gold staining

KW - Leaky surface acoustic wave (LSAW)

KW - Immunosensor

KW - MoS @Cu O-Au

KW - Gold staining

UR - https://www.scopus.com/pages/publications/85119107658

U2 - 10.3390/chemosensors9110311

DO - 10.3390/chemosensors9110311

M3 - Article

SN - 2227-9040

VL - 9

SP - 1

EP - 13

JO - Chemosensors

JF - Chemosensors

IS - 11

M1 - 311

ER -

Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this